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. Author manuscript; available in PMC: 2019 Jan 8.
Published in final edited form as: ChemMedChem. 2017 Nov 27;13(1):48–66. doi: 10.1002/cmdc.201700663

Figure 5.

Figure 5

A - Compound 1 is highly selective for GAK. SUM159 cell lysates were incubated with DMSO or the indicated concentration of 1 for 30 minutes on ice. Kinases were then affinity purified using multiplexed inhibitor beads (MIBs) and analyzed by mass spectrometry. Kinase abundance was quantified label free using MaxQuant software. Bars represent the ratio of label free quantification values for the indicated kinase in lysate treated with drug over DMSO control lysates. A dose dependent decrease in kinase ratio indicates 1 binding. B - Compound 49 is highly selective for GAK, under the same conditions as 1. C - GAK and RIPK2 enrichment from experiment with 1. D - GAK and RIPK2 enrichment from experiment with 49.