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Single-plex ND-NaME-PrO from PCR products. A. Mutation abundance obtained after single-plex ND-NaME-PrO for KRAS (c.35G>T, p.G12V) on serially decreasing mutated KRAS-containing DNA diluted in WT KRAS-containing DNA. Mutation abundance was assessed via droplet digital PCR following ND-NaME-PrO. B. Mutation enrichment obtained for KRAS mutations after ND-NaME-PrO ranged from 10-fold to 120-fold for 1% and 0.01% respectively.
