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. 2017 Oct 18;49(2):320–328. doi: 10.1016/j.bjm.2017.09.001

Fig. 1.

Fig. 1

Expression of the recombinant protein PagC and Western blot analysis. (A) Proteins were separated on 12% acrylamide gel and stained with Coomassie blue. Lane M, molecular mass markers (Blue Plus II Protein Marker, TransGen Biotech, Beijing, China); lane 1, cells carrying the vector with the insert before IPTG induction; lane 2, total cellular protein after IPTG induction; lane 3, supernatant of cells ultrasound pyrolysis products after IPTG induction; lane 4, sediment of cells ultrasound pyrolysis products after IPTG induction; lane 5, the purified protein. (B) Western blot analysis using Goat anti Rabbit HRP-IgG monoclonal antibody. Lane M, molecular mass markers (PageRuler Prest Protein Ladder, Fermentas, Shanghai, China); lane 1, western blot analysis using antiserum to recombinant protein; lane 2, western blot using pre-immune serum.