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. 2018 Apr 24;5:180068. doi: 10.1038/sdata.2018.68

Table 5. Summary of the MiSeq V1–3, V3–4, and V4 datasets.

  MiSeq V1-3 MiSeq V3-4 MiSeq V4
Read quantity A flow cell A flow cell A flow cell
No. of samples 165 165 165
Raw sequence reads (R1+R2) 22,347,080 23,654,914 29,406,440
Filtered reads (R1+R2) 21,083,242 23,427,732 29,147,046
Mean lengths of the filtered paired-end reads (bp) 243±27 262±18 250±0.7
No. of merged sequences 1,491,506 10,261,500 14,434,301
Assembly efficiency (%) 14.2±3.4 87.6±2.3 98.2±1.7
Chimera removed 1,302,039 9,166,941 13,705,970
Singleton removed 1,123,787 8,494,242 12,994,272
No. of seqs per samples (mean) 3,279 - 14,995 (6,811) 21,313 - 125,775 (51,480) 42,841 - 167,478 (78,543)
Sub-sampled 3,279 21,313 42,841
Mean read length (bp) 516±14 454±10 291