Skip to main content
. 2018 Apr 17;9:180. doi: 10.3389/fphar.2018.00180

FIGURE 3.

FIGURE 3

Araloside C protected against H/R-induced apoptosis in H9c2 cardiomyocytes. H9c2 cardiomyocytes were incubated with or without AsC for 12 h and then exposed to 6h of hypoxia and 12h of reoxygenation. (A) ΔΨm was assessed by fluorescence microscopy with JC-1 staining. (B) Apoptosis in H9c2 cardiomyocytes was analyzed by flow cytometry. (C) Quantitative analysis of mitochondrial membrane potential (ΔΨm) by plate reader. (D) Quantitative analysis of the percentages of early apoptotic cells. (E) caspase-3 activity was measured using a fluorometric assay. The values are expressed as the mean ± SD three independent experiments. ###P < 0.001 vs. Control; P < 0.05 vs. H/R group, ∗∗P < 0.01 vs. H/R group, ∗∗∗P < 0.001 vs. H/R group.