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. 2018 Apr 17;9:180. doi: 10.3389/fphar.2018.00180

FIGURE 4.

FIGURE 4

Araloside C suppressed H/R-induced apoptosis and ER stress in H9c2 cardiomyocytes. H9c2 cardiomyocytes were incubated with or without AsC for 12 h and then exposed to 6 h of hypoxia and 12 h of reoxygenation. (A,B) The expression of Bcl-2, Bax, caspase-9, caspase-3 and PARP were analyzed by western blotting, with representative bands quantified in the corresponding bar graph. (C,D) The ER stress marker GRP78 and ER stress-related apoptotic proteins (CHOP and caspase-12) were evaluated by western blot analysis. (E,F) The expression level of UPR pathway proteins were detected using western blot analysis and the relative protein expression of p-PERK to PERK, p- eIF2α to eIF2α, IRE1 and ATF6 to β-actin are expressed in the bar graphs. The values are expressed as the mean ± SD three independent experiments. #P < 0.05 vs. Control. ##P < 0.01 vs. Control. ###P < 0.001 vs. Control; ∗P < 0.05 vs. H/R group, ∗∗P < 0.01 vs. H/R group, ∗∗∗P < 0.001 vs. H/R group.