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. 2018 Apr 17;9:180. doi: 10.3389/fphar.2018.00180

FIGURE 8.

FIGURE 8

Interference of HSP90 by siRNA reversed the suppressive effect of AsC on H/R-induced ER stress. (A,B) H9c2 cardiomyocytes were transfected with control siRNA or HSP90 siRNA for 24h. These cells were then harvested, lysed, and analyzed for HSP90 protein expression levels by western blotting. (C,D) H9c2 cardiomyocytes transfected with control siRNA or HSP90 siRNA were incubated with AsC (12.5 μM) for 12 h and then subjected to 6h of hypoxia and 12 h of reoxygenation. The expression levels of ER stress-assoceated proteins were detected using western blot analysis. The relative protein expression of Bcl-2 to Bax, CHOP, caspase-12, ATF6 to β-actin, p-PERK to PERK and p- eIF2α to eIF2α were expressed in the bar graphs. The values are expressed as the mean ± SD three independent experiments. #P < 0.05 vs. Control, ##P < 0.01 vs. Control, ###P < 0.001 vs. Control; ∗P < 0.05 vs. H/R group, ∗∗P < 0.01 vs. H/R group, ∗∗∗P < 0.001 vs. H/R group.