Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 31;30(9):685–696. doi: 10.1093/protein/gzx044

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author 2017. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Fig. 6 — Analysis of pairing of light to heavy chain in the bsAb B10v5 × hu225M by LC–ESI–MS, after protein A purification and deglycosylation. The bsAbs contained either no mutation in the Fab interface (wildtype) or contained mutations in the C_H1:C_L interface (see Table II for a complete list). The V_H:V_L interface was left unmutated in all cases. The antibodies were produced by transient transfection of (A) Expi293, (B) HEK293-6E or (C) ExpiCHO. The prevalence of correctly assembled bsAb is given in percent of all detected heterodimeric IgGs and represents a mean of two (A) or three (B and C) independent transfections (Table I).