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. 2017 Jul 7;30(9):627–637. doi: 10.1093/protein/gzx034

Fig. 3.

Fig. 3

Production of anti-KLB biepitopic antibodies in the tcBsIgG format. (A) Capillary electrophoresis of co-expression of the five anti-KLB antibodies and two control antibodies (AbA, highly expressing antibody; AbB, poorly expressing antibody) as tcBsIgGs. The indicated knob half-antibody was co-expressed in a single cell with the hole half-antibody (1: AbA.hole; 2: AbB.hole; 3: 12B8.hole; 4: 2C12.hole; 5: 23B3.hole; 6: 28B7.hole; 7: 4H7.hole) and purified by protein A chromatography prior to analysis. Co-expression with a poorly expressed AbB antibody leads to poor recovery, indicating efficient heterodimerization. (B) Analytical SEC of AbA tcBsIgG (green) and 23B3/28B7 tcBsIgG (blue) after protein A affinity chromatography.