Fig. 2.

PCS activity of OsPCS variants heterologously expressed in S. pombe Δpcs under As(III) or Cd exposure. (A) Domain structures of AtPCSs and OsPCSs. The N-terminal catalytic domain (pfam05023) and C-terminal Phytochelatin_C domain of unknown function (pfam09328) are indicated. (B and C) Growth of S. pombe Δpcs harboring an empty vector pSGP72 or the cells expressing OsPCS variants when exposed to As(III) (B) or Cd (C). Data represent means with the SD of four independent replicates. (D and E) PC2 and PC3 concentrations in S. pombe Δpcs harboring an empty vector pSGP72 or the cells expressing AtPCS/OsPCS exposed to As(III) (D) or Cd (E). Data represent means with the SD of three independent replicates.