Fig. 4.

Sensitivity assay of the OsPCS1 T-DNA insertion mutant rice under As(III) or Cd stress. (A–C) The wild-type HY and the T-DNA mutant of OsPCS1 were hydroponically grown for 2 weeks without As(III) or Cd addition and then grown further for 3 weeks with or without As(III) or Cd. (A and B) Phenotypes of HY and the T-DNA seedlings (A) and leaf blades (B) after cultivation. Scale bar = 10 cm (A) or 1 cm (B). (C and D) Fresh weight of shoots (C) and roots (D) after cultivation. Data represent means with the SD of two independent experiments (n = 6–10). Asterisks indicate significant differences from the wild-type HY (*P < 0.05, **P < 0.01, ***P < 0.001; t-test).