Figure 2.

Immunoblot analysis of SLG and SRK in Brassica stigmas. A, Whole cell extracts (CE) or microsome fractions (Micro) prepared from S₁₃S_f1, ΔS-1668, scf1scf1, and SCF1SCF1 stigmas were subjected to immunoblot analysis using MAb/H8. Each lane contains 50 μg of protein. B and C, Microsome fractions isolated from wild-type (WT) stigmas (B) and from ΔS-1668 stigmas (C) were partitioned into endomembrane-enriched (Endo) and plasma membrane-enriched (PM) fractions and the blot probed with MAb/H8. Each lane contains 10 μg of protein. SRK is detected in wild-type but not in mutant stigma extracts. SLG observed in ΔS-1668 stigmas is the product of the SLG_f1 gene. The lower level of SRK in S₁₃S_f1 plants is due to the presence of only one functional copy of the SRK gene. Molecular mass markers in kD are indicated to the left of each panel.