Figure 6.

Chemical treatment of stigma microsome fractions. Equal amounts of microsome fraction obtained from Brassica stigmas were treated with 1 m H₃NO, 50 mm DTT, 8 m urea, 0.1 m Na₂CO₃, 0.2% (w/v) SDS, 1% (v/v) Triton X-100 (Tx-100), or HB (see “Materials and Methods”) and centrifuged at 100,000g for 1 h to obtain supernatant (S) and pellet (P) fractions. The fractions were subjected to electrophoresis on a 10% (w/v) polyacrylamide gel and the immunoblot was probed with MAb/H8.