Table 1.

Ingredient and analyzed composition of the experimental diets.

	P−Ca−		P−Ca+		P+Ca−		P+Ca+
Ingredient, g/kg as fed
Corn	541		541		541		541
Extracted soybean meal	400		400		400		400
Soybean oil	15		15		15		15
D,L-Methionine	2		2		2		2
Monosodium phosphate1	−		−		10.5		10.5
Sand	18		8.5		9.5		−
Limestone	10		19.5		10		19.5
Sodium chloride	1		1		1		1
Choline chloride	2		2		2		2
Sodium bicarbonate	3		3		1		1
Vitamin mix2	2		2		2		2
Mineral mix3	1		1		1		1
TiO2	5		5		5		5
	Phy−	Phy+	Phy−	Phy+	Phy−	Phy+	Phy−	Phy+
Analyzed composition
Ca, g/kg DM	5.9	6.4	9.8	10.0	6.2	6.2	10.1	9.8
Total P, g/kg DM	4.5	4.5	4.3	4.4	7.5	7.4	7.4	7.3
InsP6-P, g/kg DM	2.9	2.8	2.9	2.9	2.9	2.8	2.8	2.8
Myo-inositol, μmol/g DM	1.3	1.3	1.3	1.3	1.3	1.3	1.3	1.3
Ins(1,2,3,4,5)P54, μmol/g DM	0.5	0.6	0.5	0.5	0.6	0.5	0.5	0.5
Ins(1,2,4,5,6)P54, μmol/g DM	1.1	1.2	1.2	1.2	1.1	1.1	1.2	1.2
InsP64, μmol/g DM	15.6	15.3	15.7	15.5	15.8	15.4	15.4	15.3
Phytase Activity, FTU/kg	<50	1150	<50	1150	<50	1160	<50	1230

¹NaH₂PO₄ for use in foodstuff (Dr. Paul Lohmann GmbH KG, Emmerthal, Germany).

²Vitamin premix (Raiffeisen Kraftfutterwerke Süd GmbH, Würzburg, Germany), provided per kg of complete diet: 1,200 IU vitamin A, 3,000 IU vitamin D3, 30 mg DL-α-Tocopherylacetate, 2.4 mg vitamin K3, 3 mg vitamin B1, 6 mg vitamin B2, 6 mg vitamin B6, 30 μg vitamin B12, 50 mg nicotinic acid, 14 mg pantothenic acid, 1 mg folic acid, 0.1 mg biotin.

³Mineral mix (Gelamin, Gesellschaft für Tierernährung mbH, Memmingen, Germany), provided per kg of complete diet: 240 mg calcium from calcium carbonate, 100 mg manganese from manganese-(II)-oxide, 65 mg zinc from zinc-oxide, 25 mg iron from ferrous-(II)-carbonate, 7 mg copper from cupric-(II)-sulphate pentahydrate, 4 mg iodine from calcium iodate, 0.3 mg selenium from sodium selenite.

⁴No other InsP isomers were detected.