Table 4.

Interaction of P, Ca, and phytase on concentrations of InsP isomers (μmol/g DM) in the digesta of the crop and the proventriculus/gizzard of broiler chickens fed the experimental diets from d 15 to d 27.¹

	Dietary treatment2									P-value3
	P−Ca-		P−Ca+		P+Ca-		P+Ca+		Pooled	P	Ca	Phy	P × Ca	P × Phy	Ca ×	P × Ca
	Phy−	Phy+	Phy−	Phy+	Phy−	Phy+	Phy−	Phy+	SEM						Phy	× Phy
Crop
InsP6	14.1	4.8	13.9	5.8	14.6	4.9	14.6	4.4	0.74	n.s.	n.s.	***	n.s.	n.s.	n.s.	n.s.
Ins(1,2,4,5,6)P5	1.2	0.2	1.2	0.3	1.2	0.2	1.2	0.2	0.04	n.s.	n.s.	***	n.s.	n.s.	n.s.	n.s.
Ins(1,2,3,4,5)P5	0.8	0.2	0.7	0.2	0.6	0.4	0.7	0.3	0.04	n.s.	n.s.	***	n.s.	***	n.s.	n.s.
Ins(1,2,3,4,6)P5	0.2	n.d.4	0.2	n.d.	0.2	n.d.	0.3	n.d.	0.02	n.s.	n.s.	.	n.s.	.	.	.
Ins(1,2,5,6)P4	0.2	3.3	0.2	2.7	n.d.	4.8	<LOQ5	4.8	0.38	**	n.s.	***	n.s.	.	n.s.	.
InsP3x5	n.d.	1.0	n.d.	0.8	n.d.	1.5	n.d.	1.6	0.10	***	n.s.	.	n.s.	.	.	.
Proventriculus and gizzard
InsP6	7.2	n.d.	6.4	0.4	7.1	0.3	6.6	0.9	0.23	†	n.s.	***	n.s.	n.s.	**	.
Ins(1,2,4,5,6)P5	0.5	n.d.	0.4	n.d.	0.4	n.d.	0.4	n.d.	0.03	n.s.	n.s.	.	†	.	.	.
Ins(1,2,3,4,5)P5	0.2	n.d.	0.2	n.d.	0.2	n.d.	0.2	n.d.	0.03	n.s.	n.s.	.	n.s.	.	.	.
Ins(1,2,5,6)P4	n.d.	0.5	n.d.	1.1	n.d.	1.7	n.d.	1.9	0.21	***	**	.	*	.	.	.
InsP3x6	n.d.	n.d.	n.d.	0.4	n.d.	0.7	n.d.	0.6	0.09	†	n.s.	.	.	.	.	.

¹Data are given as treatment means; n = 7 pens.

²Calculated composition: P-, 4.1 g P/kg DM; P+, 6.9 g P/kg DM; Ca-, 6.2 g Ca/kg DM: Ca+, 10.3 g Ca/kg DM; Phy-, 0 FTU/kg; Phy+, 1,500 FTU/kg as fed.

³ P-values are indicated as follows: n.s., P ≥ 0.10; †, P < 0.10; *, P < 0.05; **, P < 0.01; ***, P < 0.001.

⁴n.d., not detectable in the majority of samples.

⁵<LOQ, not quantifiable in the majority of samples.

⁶At least one of the following isomers: Ins(1,2,6)P₃, Ins(1,4,5)P₃, Ins(2,4,5)P₃.