Table 5.

Interaction of P, Ca, and phytase on concentrations of InsP isomers (μmol/g DM) in the digesta of the duodenum/jejunum of broiler chickens fed the experimental diets from d 15 to d 27.¹

	Dietary treatment2									P-value3
	P−Ca−		P−Ca+		P+Ca−		P+Ca+		Pooled	P	Ca	Phy	P × Ca	P × Phy	Ca ×	P × Ca
	Phy−	Phy+	Phy−	Phy+	Phy−	Phy+	Phy−	Phy+	SEM						Phy	× Phy
InsP6	13.5	5.0	17.1	7.4	17.8	7.0	25.1	10.0	0.74	***	***	***	†	**	*	n.s.
Ins(1,2,4,5,6)P5	0.3d	0.3d	0.5c	0.4c,d	0.5c	0.3d	1.4a	0.7b	0.05	***	***	***	***	***	***	*
Ins(1,2,3,4,5)P5	0.6	0.9	0.8	0.9	0.8	0.9	1.1	1.6	0.11	**	**	*	*	n.s.	n.s.	n.s.
Ins(1,2,3,4,6)P5	0.2	n.d.4	0.3	n.d.	0.3	n.d.	0.4	n.d.	0.02	***	*	.	n.s.	.	.	.
Ins(1,2,5,6)P4	n.d.	1.1	n.d.	1.1	n.d.	0.9	0.3	2.8	0.20	**	***	***	***	.	.	.
Ins(1,2,3,4)P4	0.4b	0.1c	0.8a	0.1c	0.6b	0.2c	0.5b	0.4b	0.07	n.s.	*	***	†	*	n.s.	**
InsP3x5	0.3c	0.3c	0.7b	0.3c	0.5b,c	0.5b,c	0.3c	1.8a	0.10	***	***	**	*	***	**	***

¹Data are given as treatment means; n = 7 pens.

²Calculated composition: P-, 4.1 g P/kg DM; P+, 6.9 g P/kg DM; Ca-, 6.2 g Ca/kg DM: Ca+, 10.3 g Ca/kg DM; Phy-, 0 FTU/kg; Phy+, 1,500 FTU/kg as fed.

³ P-values are indicated as follows: n.s., P ≥ 0.10; †, P < 0.10; *, P < 0.05; **, P < 0.01; ***, P < 0.001.

⁴n.d., not detectable in the majority of samples.

⁵At least one of the following isomers: Ins(1,2,6)P₃, Ins(1,4,5)P₃, Ins(2,4,5)P₃.

^a–dMeans within a row not showing a common superscript differ (P < 0.05).