Table 6.

Interaction of P, Ca, and phytase on concentrations of InsP isomers (μmol/g DM) in the digesta of the terminal ileum of broiler chickens fed the experimental diets from d 15 to d 27.¹

	Dietary treatment2									P-value3
	P−Ca−		P−Ca+		P+Ca−		P+Ca+		Pooled	P	Ca	Phy	P × Ca	P × Phy	Ca ×	P × Ca
	Phy−	Phy+	Phy−	Phy+	Phy−	Phy+	Phy−	Phy+	SEM						Phy	× Phy
InsP6	17.4	5.6	20.7	10.3	26.7	5.7	35.6	10.6	1.29	***	***	***	n.s.	***	n.s.	n.s.
Ins(1,2,4,5,6)P5	0.3c	0.4c	0.7b	0.7b	0.7b	0.3c	2.0a	0.8b	0.09	***	***	***	***	***	*	*
Ins(1,2,3,4,5)P5	1.1	1.2	1.0	1.6	1.6	0.8	1.9	2.0	0.25	*	*	n.s.	n.s.	n.s.	†	n.s.
Ins(1,2,3,4,6)P5	0.5	n.d.4	0.4	n.d.	0.7	n.d.	0.8	n.d.	0.04	***	n.s.	.	†	.	.	.
Ins(1,2,5,6)P4	n.d.	1.4	n.d.	1.7	0.2	0.9	0.6	4.9	0.42	*	**	***	**	.	***	.
Ins(1,2,3,4)P4	0.8	n.d.	1.1	0.3	1.1	0.2	0.9	0.5	0.11	n.s.	†	***	†	*	*	.
Ins(1,5,6)P3	<LOQ5	0.2	<LOQ	<LOQ	0.2	0.2	0.2	0.2	0.02	n.s.	n.s.	n.s.	.	.	n.s.	.
InsP3x6	0.6b,c	0.4c	1.1b	0.4c	0.7b,c	0.6b,c	0.5b,c	3.3a	0.19	***	***	n.s.	*	***	**	***
Ins(1,2)P2	1.2b,c	0.4d	2.0b	0.5c,d	1.1c,d	2.0b	0.6c,d	3.1a	0.25	**	*	n.s.	n.s.	***	n.s.	**
InsP1x7	1.3	2.2	1.6	2.2	1.0	3.8	0.9	3.2	0.37	n.s.	n.s.	***	n.s.	**	n.s.	n.s.

¹Data are given as treatment means; n = 7 pens.

²Calculated composition: P-, 4.1 g P/kg DM; P+, 6.9 g P/kg DM; Ca-, 6.2 g Ca/kg DM: Ca+, 10.3 g Ca/kg DM; Phy-, 0 FTU/kg; Phy+, 1,500 FTU/kg as fed.

³ P-values are indicated as follows: n.s., P ≥ 0.10; †, P < 0.10; *, P < 0.05; **, P < 0.01; ***, P < 0.001.

⁴n.d., not detectable in the majority of samples.

⁵<LOQ, not quantifiable in the majority of samples.

⁶At least one of the following isomers: Ins(1,2,6)P₃, Ins(1,4,5)P₃, Ins(2,4,5)P₃.

⁷At least one of the following isomers: Ins(1)P₁, Ins(2)P₁.

^a–dMeans within a row not showing a common superscript differ (P < 0.05).