Fig. 2. (A) GAPDH transcripts are detected with ON-N3-Quasar 570 using the direct staining approach. (B) Quasar 570 is cleaved by TCEP. (C) Fluorescence intensity profiles corresponding to the yellow lines positions in (A) and (B). (D) MKI67 transcripts are detected with ON-N3-Cy5 using the indirect staining approach. (E) Cy5 is cleaved by TCEP. (F) Fluorescence intensity profiles corresponding to the yellow lines positions in (D) and (E). (G) Genomic locus 4p16.1 is stained with ON-N3-Cy5 using the indirect staining approach. (H) Cy5 is cleaved with TCEP. Cell nuclei are stained with DAPI (blue) in (G) and (H). (I) Fluorescence intensity profiles corresponding to the yellow lines positions in (G) and (H). Scale bars, 5 μm.
