Fig. 5. (A) MKI67 and GAPDH transcripts are detected in the same cell using the indirect staining approach with ON-N3-Quasar 570 (green) and ON-N3-Cy5 (red), respectively. (B) The mean copy numbers of MKI67 and GAPDH transcripts per cell measured by RNA FISH with BoCFO and conventional RNA FISH (P > 0.2; error bars, s.d.; n = 30 cells). The y axe in (B) is on a logarithmic scale. Scale bars, 5 μm.
