Figure 7.

Effects of compound 8f on the LPS-induced activation of NF-κB and MAPK signaling pathway. MPMs pretreated with vehicle or compound 8f (10 µM) for 30 min followed by incubation with LPS (0.5 µg/mL) for 20 min. The protein levels of IκB-α, p-JNK, JNK, p-ERK, ERK, and GAPDH were measured by immunoblot analysis. Statistical significance relative to the LPS group was indicated, n≥5, ^*P<0.05, ^**P<0.01.

Abbreviations: LPS, lipopolysaccharide; MPMs, murine primary macrophages; CON, control group.