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. 2000 Sep;124(1):321–330. doi: 10.1104/pp.124.1.321

Figure 5.

Figure 5

Distribution of marker enzymes from fractions derived from a nonaqueous density gradient of freeze-dried, sonicated celery leaves. Typically, distribution of α-mannosidase (EC 3.2.1.24) is in the vacuole, NADP-dependent reversible glyceraldehyde-3-P dehydrogenase (GAP-DH, EC 1.2.1.13) is in the chloroplast, and PEP carboxylase (PEPcase, EC 4.1.1.31) is in the cytosol. Recoveries of the marker enzymes were in the range of 93% to 106% based on total activities in the unfractionated materials.