Table I.
Step | Activity | Protein | Specific Activity | Purification | Yield |
---|---|---|---|---|---|
μmol min−1 | mg | μmol min−1 mg−1 | -fold | % | |
Homogenate | 229.8 | 5,530 | 0.042 | NAa | NA |
(NH4)2SO4 | 200.4 | 914 | 0.22 | 5 | 87 |
Acetone | 164.8 | 352 | 0.47 | 11 | 72 |
DEAE-Sepharose | 144.7 | 29 | 4.93 | 119 | 63 |
Sephadex-200 | 101.3 | 8.2 | 12.4 | 299 | 44 |
Affi-Blue | 68.9 | 2.25 | 30.7 | 738 | 30 |
NA, Not applicable.