Coomassie Brilliant Blue-stained polypeptide patterns of enriched-thylakoid (A) and soluble (B) extracts from E. chlorotica over a 9-month period cultured in the absence of algae and from V. litorea. Lane 1, Molecular mass markers in kD; lanes 2 through 10, E. chlorotica months 1 through 9 in culture; lane 11, V. litorea. Sample size, 50 μg protein per lane. B, Star, 52-kD Rubisco LS protein; arrowheads, 38-, 36-, and 26-kD polypeptides, which predominate in the alga, but are absent or greatly reduced in the sea slugs.