Figure 4.
De novo synthesis of enriched-thylakoid (A) and soluble (B) polypeptides by E. chlorotica over a 9-month culture period in the absence of algae, and by V. litorea filaments. Animals and algae were labeled with [35S]Met and fractionated as described in “Materials and Methods.” Lane 1, V. litorea; lanes 2 through 5, E. chlorotica labeled at 3, 5, 7, and 9 months of culture. A, lane 3, asterisk, 32-kD D1 protein; lane 5, asterisk, four major membrane polypeptides synthesized by the sea slugs after 9 months symbiotic association; B, lanes 1 and 4, asterisk, 52-kD Rubisco LS protein in algal and sea slug extracts, respectfully. Samples were loaded on an equal radioactivity basis (100,000 dpm/lane).