V. litorea ctDNA digested with PstI and electrophoresed through 1% (w/v) agarose. Fragment sizes (in kb) in descending order are 19.5, 15.6, 11.8, 10.6, 7.9 × 2, 6.8, 6.3, 5.7, 4.8, 4.1, 3.7, 3.6, 3.3, 2.9, 2.2, 1.7, and 0.7 (not visible on picture) for a total chloroplast genome size of 119.1 kb.