Figure 1.

RT-qPCR shows that treatment of PA-1 cells with etoposide (ETO) leads on day 3 to bias of enhanced expression of the conventional and  alternative splicing of POU5F1/Oct4 which is coupled to downregulation of the Oct4A partners in the pluripotency network Sox2 and Lin28. The discorrelation between the Oct4A, Sox2 and Lin28 is corrected, while the splicing variant Oct4B drops down, in the survival cells on day 20. Average numbers from two independent experiments are presented. Relative mRNA levels are shown after normalization against four housekeeping genes for every sample (for details see Material and Methods). mRNA levels in non-treated (NT) cells are arbitrary set as 1 and shown as folds after ETO treatment. Statistical significance is shown by a two-tailed T-test:*p<0.05; **p<0.01.