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. 2018 Jan 31;17(3):300–308. doi: 10.1080/15384101.2017.1403686

Figure 2.

Figure 2.

UHRF1 knockdown increases UbcH8 expression while its over-expression decreases UbcH8 expression. (A) The mRNA levels of UHRF1 and UBE2L6 were measured by Real time-PCR in RPE1 cells expressing E7 (E7) and HeLa cells after transfected with siRNAs targeting UHRF1. (B) The protein levels of UHRF1 and UbcH8 were measured by Western blot in E7 cells after transfected with siRNAs targeting UHRF1. Right panel, quantification of UHRF1 and UbcH8 protein expressions. (C) The protein levels of UHRF1 and UbcH8 were measured by Western blot in HeLa cells after transfection with plasmid encoding UHRF1. Right panel, quantification of UHRF1 and UbcH8 protein expressions. N, non-targeting siRNA; si1, siRNA-1 targeting UHRF1; si2, siRNA-2 targeting UHRF1. *, p < 0.05; **, p < 0.01.