Figure 5.

UHRF1 and UbcH8 regulate apoptosis. (A) RPE1-E7 cells were transfected with siRNAs targeting UHRF1 and treated with Etoposide. Cells were analyzed by flow cytometry after fixation and PI stain. Cells with sub-G1 DNA content were gated and quantified. (B) RPE1-E7 cells were transfected with plasmid encoding UbcH8, treated and analyzed as above. (C) RPE1-E7 cells were transfected with siRNAs targeting UHRF1 and treated with Etoposide. Cells were double stained with annexin V-FITC/PI and analyzed by flow cytometry. Cells that stained positive for annexin V-FITC and negative for PI were undergoing early stage apoptosis; cells that stained positive for both annexin V-FITC and PI were in the late stage of apoptosis or undergoing necrosis. Percentage of apoptotic cells (annexin V-FITC positive) was quantified. (D) RPE1-E7 cells were transfected with plasmid encoding UbcH8, treated and analyzed as above. Data of 3 independent experiments were summarized. *, p < 0.05 **, p < 0.01. NC, negative control.