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. 2000 Sep;124(1):343–354. doi: 10.1104/pp.124.1.343

Figure 4.

Figure 4

Changes in ABA and ethylene levels, and in PaNCED1, PaNCED2, and PaNCED3 transcript accumulation during the course of avocado fruit ripening. A, Analysis of ABA and ethylene levels plotted as a function of days of ripening. B, Northern analysis of NCED gene expression in the same fruits. Total RNA (30 μg per lane) was isolated from fruit, separated by gel electrophoresis, and blotted onto nylon membranes. The same blot used for analysis of PaNCED1 was stripped and reprobed with probes against PaNCED2 and subsequently PaNCED3 and 17S rDNA. The specific probes used for the PaNCED genes are described in “Materials and Methods.” The 17S rDNA probe was used as a loading control.