Figure 2.

Differences at lateral contact within the MT between mammalian and yeast tubulin. (A) Cα traces for the yeast tubulin model of two adjacent tubulin dimers. The boxes correspond to the regions displayed in (B) and (C). Superposition of the yeast atomic model [34] aligned with mammalian tubulin [12] around the lateral contacts for β-tubulin (B) and α-tubulin (C). Residue differences between yeast and mammalian tubulin near the lateral interface are indicated. Yeast models shown in darker shades (α-tubulin in green, β-tubulin in blue). Labeled residues reference mammalian tubulin residue first. Contacts between β-tubulins are similar, while in α-tubulin the loops between helix-H1 and β-strand-S2, and between β-strand-S7 and helix-H9 (C-terminal region of the M-loop) adopt different conformations.