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. 2000 Sep;124(1):369–378. doi: 10.1104/pp.124.1.369

Figure 4.

Figure 4

Primer-directed RNaseH digestion of Abp1 mRNA. Total RNA was incubated with RNaseH and ZMAUX3435A (lanes 1–5), ZMAUX3435A only (lane 8), or RNaseH only (lane 9), or was untreated (lanes 6 and 7), followed by northern analysis. The membrane was probed with EX1 and subjected to autoradiography (A), and then probed with 3PE and re-exposed (B). Lane 1, Z. mays subsp. mays cv Seneca Horizon; lanes 2 and 6, Z. mays subsp. parviglumis; lanes 3 and 7, Z. mays subsp. mexicana; lanes 4 and 8, Z. diploperennis; lanes 5 and 9, Z. luxurians. The asterisks indicate the positions of the intact Abp1 transcript (*), the 5′ fragment (**), and the 3′ fragment (***).