Figure 4.

Rilmenidine stimulates macroautophagy in H9 human embryonic stem cell-derived spinal motor neurons. Photomicrographs of motor neuron spheres stained for (A) HB9 and (B) ISL1, and dissociated mature motor neurons stained with (C) CHAT and (D) TUBB3 antibodies. (E) Immunoblot analysis of macroautophagy markers in induced motor neurons transiently transfected with wild-type SOD1 and treated with rilmenidine (Ril) for 24 h post-transfection. Quantification of (F) LC3-II and (G) SQSTM1 protein levels from immunoblots normalized to untreated cells. Data represent mean ± SD, n = 5 independent experiments, *p<0.05 compared to untreated cells using an unpaired t-test.