Figure 6.
Rilmenidine treatment increases autophagosome number in spinal motor neurons of SOD1G93A mice. (A) LC3 immunohistochemical analysis in lumbar spinal cords of vehicle- and rilmenidine-treated SOD1G93A mice. In vehicle-treated mice, there is diffuse distribution of autophagosomes in the cytoplasm of RBFOX3/NeuN-positive motor neurons. In rilmenidine-treated mice, there is accumulation of autophagosomes (arrows) in motor neurons. (B) LAMP2 immunohistochemical analysis in lumbar spinal cords of vehicle- and rilmenidine-treated SOD1G93A mice. In vehicle- and rilmenidine-treated mice, lysosomes (arrows) are distributed in the cytoplasm of motor neurons. Quantification of (C) LC3-positive autophagosomes and (D) LAMP2-positive lysosomes in motor neurons. Data represent mean ± SD, n = 3 mice per group, *p<0.05 compared to vehicle-treated mice using an unpaired t-test.