Table 5.
Antibodies used for the immunohistochemical analysis.
| Antibodies |
Clone |
Dilution |
Source |
Heat-induced Antigen Retrieval |
Incubation |
Positive style |
Positive control |
Cut-off values (%) |
| PDL1 | Rabbit monoclonal | Prediluted | Ventana Medical Sstems,Inc,ready-to-use ,code:263 | 100°C | 37°C | membrane staining | placenta | ≥25 |
| 30 min | 16 min | |||||||
| PD1 | Mouse monoclonal | Prediluted | OriGene, MRQ-22 | 100°C | 37°C | plasmatic staining | tonsile | ≥1 |
| 30 min | 16 min | |||||||
| CK14 | Mouse monoclonal | Prediluted | Novocatra™,Leica Biosystem, LL002 | 100°C | 37°C | plasmatic staining | Head and neck cancer | ≥1 |
| 30 min | 16 min |