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. 2018 Jan 29;14(3):368–384. doi: 10.1080/15548627.2017.1413521

Figure 4.

Figure 4.

Identification of Atg37- and Pex3-binding regions in Atg30. (A) Schematic of truncated forms of Atg30 used to map Atg37- and Pex3- binding sites in Atg30. (B) Atg37 interacts with Atg30 constructs containing the Atg30-5 fragment of Atg30 in a Y2H assay; AD fusions of truncated forms of Atg30 (Atg30-1-6) were used with previously described BD-Atg37(1-278).12 (C) Pex3 interacts with the Atg30-5 fragment of Atg30 in a Y2H assay. The listed AD-Atg30 fusions were used with the previously described BD-Pex3 and BD-Pex3m fusions.12,13 (D) AD-Atg30-5 and AD-Atg30-6 interact with BD-Pex3 as strongly as does full-length AD-Atg30 in a Y2H assay.