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. 2018 Feb 5;19(5):364–372. doi: 10.1080/15384047.2018.1423915

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Figure 4. — Bioinformatics analysis and in vitro experiments with silenced COL11A1 and negative control. (A) Expression levels of COL11A1 in 6 ESCC cell lines were measured through western blotting assay. Cells infected with shCOL11A1 showed remarkable silencing efficiency. (B) and (C) ESCC cells with inhibition of COL11A1 expression resulted in decreased growth rate (p = 0.0035) and impaired colony formation ability (p = 0.0126). (D) Correlation analysis revealed strong connections between COL11A1 and mesenchymal markers (spearman's correlation). (E) GSEA results revealed an enhancive effect of COL11A1 in cancer invasion and EMT. (F) Increased epithelial markers and decreased mesenchymal markers were found in COL11A1-knockdown cells. (G) Transwell assay showed that cells with down-regulated COL11A1 could weaken migration (p = 0.0008) and invasion (p<0.0001) capacity. (H) A delay of wound closure was observed in COL11A1-knockdown cells (p = 0.0062). Triplicate independent experiments were performed. * p<0.05, ** p<0.01, *** p<0.001.