Figure 6.
Functional analysis of ATI3 genes in plant resistance to B. cinerea. Six-wk-old Col-0 WT, ati3 single, double and triple mutants and ati3a mutant complemented with WT ATI3A and the mutant gene encoding ATI3AW260A were inoculated by spraying spore suspension at a density of 2.5 × 105 spores/mL and kept at high humidity. Photographs of representative plants were taken 4 d after inoculation (A). Total RNA was isolated from the plants 4 d after inoculation and the transcript levels of the B. cinerea actA gene were determined using qRT-PCR with the Arabidopsis AT3G18780/ACT2 gene as an internal control (B). Data represent means and standard errors (n = 5). According to Duncan's multiple range test (P = 0.05), means of B. cinerea actA gene transcript levels do not differ significantly if they are indicated with the same letter.