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. 2018 Apr 6;9(26):18002–18017. doi: 10.18632/oncotarget.24649

Figure 5. Down-regulation of CD95 expression by siRNA reduced plasma-induced cell apoptosis.

Figure 5

(A) CD95 expression detected by flow cytometry, real-time PCR and western blotting after siRNA-mediated knockdown for 48 h. IgG is the isotype control. Con indicates transfection of control scramble siRNA compared to CD95-targeting siRNA. (B, C) CD95 expression (B) and corresponding cell viability (C) 24 h after plasma treatment for 0.5 min, 1 min and 2 min. Con- and Con+ groups were transfected with control scramble siRNA and treated without or with He+O2 plasma. The siRNA+ group represents cells treated with He+O2 plasma after knockdown of CD95 by siRNA. (D) Cell apoptosis was detected 24 h after He+O2 plasma treatment for 1 min following transfection. * indicates p<0.05.