Figure 6. Extracellular and intracellular ROS, cell viability and CD95 expression were measured after He+O₂ plasma treatment with ROS scavenger (NAC).

(A) Extracellular ROS level detected with a microplate reader at 0 h, 24 h and 48 h after plasma treatment for 0.5, 1 and 2 min with or without NAC. (B) Intracellular ROS level detected with a flow cytometer 24 h after plasma treatment without (above) or with (below) NAC. (C) Cell viability measured by CellTiter-Glo assay at 0 h, 24 h and 48 h after plasma treatment for 0.5, 1 and 2 min with or without NAC. (D) CD95 expression was detected by flow cytometry (left) and real-time PCR (middle) 24 h after He+O₂ treatment and by western blot (right) 48 h after plasma treatment with or without NAC.