Figure 7. Involvement of p53 in CD95-mediated cell apoptosis by He+O₂ plasma treatment.

(A) Analysis of cell viability after plasma treatment for 0.5, 1 and 2 min with or without NAC or PFT-α (p53 inhibitor). (B) Western blot analysis of phospho-p53, caspase3/8/9 and CD95 expression after plasma treatment. NAC and PFT-α indicates that the ROS scavenger and p53 inhibitor were added respectively before plasma treatment for 2 min. (C) Interaction of p53 and CD95 promoter region in response to plasma treatment. The top panel shows the illustration of the CD95 promoter region and p53 binding site. The bottom panel shows the results of ChIP assay for p53 and CD95 promoter detected by RT-PCR and real-time PCR. ^* indicates p<0.05.