Figure 1. Effect of 2HF alone and in combination with RLIP76 inhibition in breast cancer survival and drug-resistance.

(A-B) The effects of anti-RLIP76 IgG (10 μg/ml conc.) and 2HF (5 and 10 μM conc.) and their combination on the survival of MCF7 (A) and MDA-MB-231 (B) cells conducted using MTT assay. (C-D) Effects of anti-RLIP76 IgG (10 μg/ml) and 2HF (10 and 20 μM) and their combination on the transport of ¹⁴C-DOX in purified reconstituted vesicles prepared from MCF7 (C) and MDA-MB-231 (D) cells performed by rapid-filtration technique using 250 ng RLIP76 protein per 30 μl reaction mixture.