Table 1. Plasmids used in this study.
Name | Insert/property | Reference |
---|---|---|
Expression vectors | ||
pcDNA3.1-Mcl-1 | MCL-1 cDNA without 3′UTR Flag-Tag (8aa) between START codon and MCL-1 cDNA |
[70] |
pCMV-CyclinD1 | CyclinD1 cDNA without 3′UTR | [71] (Addgene # 19927) |
Luciferase reporter plasmids | ||
pMIR-Report-MYCN-UTR-WT | MYCN-3′UTR reporter plasmid | [69] |
pMIR-Report-MYCN-UTR-MUT | Mutated MYCN-3′UTR reporter plasmid | this study |
pGL4.75[hRluc/CMV] | Renilla luciferase reporter | Promega |