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. 2018 Apr 6;9(26):18309–18326. doi: 10.18632/oncotarget.24822

Figure 1. Assessment of EndoG's function in initiation of mitochondrial OH replication and mitochondrial transcription.

Figure 1

(A) Organization of the human mitochondrial genome. Recognition sites of mREP and mTRANS probes for FISH labelling with mTRIP are indicated with red and green boxes, respectively. HSP and LSP - heavy - and light-strand promoter, OH - origin of heavy strand replication, NCR - non-coding region (adopted from [7]). (B) Representative confocal microscopic images of HeLa cells stained with DAPI and MitoTracker DR (250-1000 nM, 1 h) and labelled with mREP and mTRANS probes 48 h after EndoG knockdown with siRNA. Scale bars = 10 μm. Fluorescence-based quantification of (C) mREP (initiation of mtDNA replication near OH) and (D) mTRANS (mitochondrial transcription); n = 196–218 cells from 2 independent experiments. Data are expressed as mean ± SEM (****P < 0.0001; non-parametric Mann-Whitney test for unpaired samples). (E) Western blot analysis to validate efficient knockdown of EndoG.