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. 2018 Apr 6;9(26):18309–18326. doi: 10.18632/oncotarget.24822

Figure 6. Impact of EndoG on expression of factors involved in mitochondrial genome replication, BER or fusion.

Figure 6

HeLa cells were grown in galactose medium (72 h) and transfected with control nsRNA or siEndoG and cultivated for 48 h. (A) Left panel: representative image of Western blot analysis of POLγ expression; right panel: quantification of Western blot analysis. n = 4 independent experiments. (B–F) Representative immunofluorescence microscopic images (left panel) and fluorescence-based quantification (right panel) of protein levels of (B) TWINKLE n = 120, (C) mtSSB n = 110–127, (D) APE1 n = 157–171, (E) MFN2 n = 157–171 or (F) OPA1 n = 103–141 cells from 2 independent experiments each. Data are expressed as mean ± SEM (ns, not significant; *P < 0.05; ****P < 0.0001; non-parametric Mann-Whitney test for unpaired samples); Scale bars = 10 μm.