Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Apr 6;9(26):18454–18479. doi: 10.18632/oncotarget.24862

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © 2018 Greer et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PMC Copyright notice

(A) Cell viability assays comparing the effect of ONC201 between MB231 and HFF cells in glucose medium. Results are shown as mean +/− SEM of 3 independent experiments. (B) ATP assays comparing the effect of ONC201, oligomycin, and metformin between MB231 and HFF cells in glucose medium. Results are shown as mean +/− SEM of 3 independent experiments. In both A) and B) ^**p<0.01, ^****p<0.0001, two-way ANOVA. (C) Cell viability assays (top) and ATP assays (bottom) of HFF cells treated with ONC201 and other mitochondrial targeting drugs in glucose or galactose containing medium. Results are shown as mean +/− SEM of multiple independent experiments. ^****p<0.0001, two-way ANOVA. (D) TEM image of HFF cells treated with ONC201 (5μM) in glucose medium for indicated times. (E) Confocal microscopy image of HFF cells treated with DMSO (Ctl.) or ONC201 (5μM) for 24 h in glucose medium, and co-stained with MitoTracker Deep Red, PicoGreen and Hoechst. Scale bar = 20μm. (F) Quantitative analysis of mtDNA copy number in HFF cells treated with ONC201 5μM for indicated times in glucose medium. Results are shown as mean +/− SEM of 3 independent experiments. ^*p<0.05, ^***p<0.001 (t-test vs. time zero). (G) XF analyzer results comparing basal level of OCR/ECAR of MB231, SKBR3 and HFF cells (in glucose medium). Cell density was 100% confluent on the day of OCR/ECAR measurement. Experiments were repeated multiple times. (MB231: n=5, SKBR3: n=2, HFF: n=2).

(A) Cell viability assays comparing the effect of ONC201 between MB231 and HFF cells in glucose medium. Results are shown as mean +/− SEM of 3 independent experiments. (B) ATP assays comparing the effect of ONC201, oligomycin, and metformin between MB231 and HFF cells in glucose medium. Results are shown as mean +/− SEM of 3 independent experiments. In both A) and B) ^**p<0.01, ^****p<0.0001, two-way ANOVA. (C) Cell viability assays (top) and ATP assays (bottom) of HFF cells treated with ONC201 and other mitochondrial targeting drugs in glucose or galactose containing medium. Results are shown as mean +/− SEM of multiple independent experiments. ^****p<0.0001, two-way ANOVA. (D) TEM image of HFF cells treated with ONC201 (5μM) in glucose medium for indicated times. (E) Confocal microscopy image of HFF cells treated with DMSO (Ctl.) or ONC201 (5μM) for 24 h in glucose medium, and co-stained with MitoTracker Deep Red, PicoGreen and Hoechst. Scale bar = 20μm. (F) Quantitative analysis of mtDNA copy number in HFF cells treated with ONC201 5μM for indicated times in glucose medium. Results are shown as mean +/− SEM of 3 independent experiments. ^*p<0.05, ^***p<0.001 (t-test vs. time zero). (G) XF analyzer results comparing basal level of OCR/ECAR of MB231, SKBR3 and HFF cells (in glucose medium). Cell density was 100% confluent on the day of OCR/ECAR measurement. Experiments were repeated multiple times. (MB231: n=5, SKBR3: n=2, HFF: n=2).