Figure 3. Co-culture experiments using mesothelioma cell lines and fibroblasts.
(A) Proliferation assay. NHLF promoted proliferation of mesothelioma cells. (B, C) Migration/Invasion assay. NHLF promoted migration and invasion of mesothelioma cells. (D) Western blot analysis. CTGF expression in ACC-MESO-4 was significantly increased upon co-culture. NHLF also expressed CTGF. N = 3 for each group in (A)-(C) and N = 4 in (D); means ± SEM, *P < 0.05, **P < 0.01, ***P < 0.005. NHLF, normal human lung fibroblast.