Figure 4.

(A) Representative immunocytochemistries of H9c2 cells cultured in normal glucose (Normal glu) or 33 mM glucose (High glu) and labelled with KCNQ1 antibody. Cells were exposed to DMSO 0.05 µM and BF-5 m 0.01–0.025–0.05 µM. Representative microscopic fields for each treatment are shown and accordingly, the percentage of KCNQ1-positive cells is represented in the graph. (B) The results are expressed as mean ± S.E.M. of the percentages of positive cell / total cell counted in each analyzed field for each treatment. ^**P < 0.01 Normal glu; °°P < 0.01 vs High glu. 40× magnification.