Figure 6. Representative merged images of MitoTracker labeling of mitochondria (red), immunofluorescence with KCNQ1 antibody (green) and counterstaining of cell nuclei with Hoechst 33258 (blue) in H9c2 cells cultured in normal glucose (Normal glu) or 33 mM glucose (High glu) and exposed to DMSO 0.05 µM and BF-5m 0.01–0.025–0.05 µM.

Merged images did not demonstrate a significant overlap between mitochondria and KCNQ1 in H9c2 exposed to normal or high glucose medium and receiving DMSO 0.1 µM or BF-5 m 0.01–0.025–0.05 µM pretreatment. 40× magnification.