Figure 3. Wound healing assay to analyze endothelial cell migration.

(A) Representative photographs taken at time 0 and 7 h. The dashed lines show the initial area without cells. Controls are untreated HMEC endothelial cells and “negative controls” are cells with only the transfection reagents added. (B) Quantitative analysis of wound healing data for HMEC cells. Data represent the means ± SD of three independent experiments and they are given as ratios (dividing the signals in control samples by the corresponding siRNA experiment signals).