Figure 8. Blocking of SOD3 with antiSOD3 inhibits in vitro and ex vivo angiogenesis.

(A) Inhibition of “tubular-like” structures within a 3D matrix. Representative photographs of control and antibody-treated endothelial cells within a 3D type I collagen matrix. (B) Inhibition of endothelial cells to elastin. Representative photographs of control and antibody-treated, calcein-labeled endothelial cells adhered to elastin and quantitative analysis of data. (C) Quantification of increasing number of invading cells with time in the invasion assay. (D) Inhibition of ex vivo angiogenesis. Representative photographs of control and antibody-treated rat aortic ring explants after 14 days of culture and quantitative analysis of data. Data represent the means ± SD of three independent experiments. Symbols indicate significant differences between control and treated cells (^*p <0.05, ^**p<0.01, ^***p<0.005).